A simple, reproducible, sensitive and cost effective stability indicating micellar liquid chromatographic (MLC) method has been developed for determination of Tacrolimus (TCR) in bulk and capsule dosage form. The analysis was carried out on Thermo Scientific Hypersil BDS cyano RP column (250 mm x 4.6 mm, 5 µm), using acetonitrile: methanol: water: 0.1 N H2SO4 (15:10:70:5 v/v/v/v) containing sodium lauryl sulphate (0.01 M) as a mobile phase at a flow rate of 1.5 ml/min and UV detection at 215 nm. Beer's law was found to obey in the concentration range of 50-150 µg/ml with correlation coefficient of 0.9981. The retention time for TCR was found to be 2.61 min. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.1383 µg/ml and 0.4192 µg/ml respectively. The % relative standard deviations for intra-day and inter-day precision studies were found to be 0.08-0.18 and 0.31-0.57, respectively. The % recovery of TCR was found to be in the range 98.42 to 100.34. The proposed method was applied successfully for the determination of TCR in bulk and its capsule dosage form.
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